Journal: Developmental neurobiology
Article Title: The immunoglobulin-like superfamily member IGSF3 is a developmentally regulated protein that controls neuronal morphogenesis.
doi: 10.1002/dneu.22412
Figure Lengend Snippet: Fig. 7. IGSF3 negatively regulates the size of TSPAN7 clusters in cerebellar granule cells. A. IGSF3/TSPAN7 complex in the mouse brain detected by IGSF3 affinity-purification from P8 cerebella. 4% Total input lysate (IN), 4% flow through (FT), 50% Immunoprecipitates (IP); control: beads coated with IgG
Article Snippet: Primary antibodies Sheep anti-IGSF3 (1:750 for brain sections, 1:400 for fixed cells, R&D Systems), rabbit anti-IGSF3 (for Western blotting, 1:1000, Sigma), mouse anti-Calbindin (1:5000, Swant), guinea pig anti-GLAST (1:7000, Chemicon), anti-PAX6 (Covance, 1:300), anti-NCAM L1 (1:500, Millipore), guinea pig antiVglut1 (1:5000, Millipore), anti-TSPAN7 (1:500, a kind gift of Dr. Passafaro), anti-TAG1 (1:2, kindly provided by Pr.
Techniques: Affinity Purification, Control